cells assemble invadopodia-like structures and invade into matrigel in a matrix metalloprotease dependent manner in the circular invasion assay细胞组装invadopodia-like结构和入侵到基底膜基质在循环矩阵metalloprotease依赖的方式入侵检测.pdfVIP

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cells assemble invadopodia-like structures and invade into matrigel in a matrix metalloprotease dependent manner in the circular invasion assay细胞组装invadopodia-like结构和入侵到基底膜基质在循环矩阵metalloprotease依赖的方式入侵检测.pdf

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cells assemble invadopodia-like structures and invade into matrigel in a matrix metalloprotease dependent manner in the circular invasion assay细胞组装invadopodia-like结构和入侵到基底膜基质在循环矩阵metalloprotease依赖的方式入侵检测

Cells Assemble Invadopodia-Like Structures and Invade into Matrigel in a Matrix Metalloprotease Dependent Manner in the Circular Invasion Assay Xinzi Yu, Laura M. Machesky* The Beatson Institute for Cancer Research, Glasgow University College of Medical Veterinary and Life Sciences, Glasgow, United Kingdom Abstract The ability of tumor cells to invade is one of the hallmarks of the metastatic phenotype. To elucidate the mechanisms by which tumor cells acquire an invasive phenotype, in vitro assays have been developed that mimic the process of cancer cell invasion through basement membrane or in the stroma. We have extended the characterization of the circular invasion assay and found that it provides a simple and amenable system to study cell invasion in matrix in an environment that closely mimics 3D invasion. Furthermore, it allows detailed microscopic analysis of both live and fixed cells during the invasion process. We find that cells invade in a protease dependent manner in this assay and that they assemble focal adhesions and invadopodia that resemble structures visualized in 3D embedded cells. We propose that this is a useful assay for routine and medium throughput analysis of invasion of cancer cells in vitro and the study of cells migrating in a 3D environment. Citation: Yu X, Machesky LM (2012) Cells Assemble Invadopodia-Like Structures and Invade into Matrigel in a Matrix Metalloprotease Dependent Manner in the Circular Invasion Assay. PLoS ONE 7(2): e30605. doi:10.1371/journal.pone.0030605 Editor: Maddy Parsons, King9s College London, United Kingdom Received October 18, 2011; Accepted December 25, 2011; Published February 8, 2012 Copyright: 2012 Yu, Machesky. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and