characterisation of australian mrsa strains st75- and st883-mrsa-iv and analysis of their accessory gene regulator locus描述澳大利亚的mrsa菌株st75u2014u2014st883-mrsa-iv和分析他们的附属基因监管机构轨迹.pdfVIP

Characterisation of Australian MRSA Strains ST75- and ST883-MRSA-IV and Analysis of Their Accessory Gene Regulator Locus 1 2 1 ¨ 3 4 5 Stefan Monecke *, Hanna Kanig , Wolfram Rudolph , Elke Muller , Geoffrey Coombs , Helmut Hotzel , Peter Slickers3, Ralf Ehricht3 1 Faculty of Medicine ‘‘Carl Gustav Carus’’, Institute for Medical Microbiology and Hygiene, Technical University of Dresden, Dresden, Germany, 2 Department for Applied Microbiology, Institute for Microbiology, Technical University of Dresden, Dresden, Germany, 3 Alere Technologies GmbH, Jena, Germany, 4 PathWest Laboratory ¨ Medicine-WA, Royal Perth Hospital, Perth, West Australia, Australia, 5 Friedrich-Loffler-Institute, Institute of Bacterial Infections and Zoonoses, Jena, Germany Abstract Background: Community-acquired methicillin-resistant Staphylococcus aureus have become a major problem in Australia. These strains have now been isolated throughout Australia including remote Indigenous communities that have had minimal exposure to healthcare facilities. Some of these strains, belonging to sequence types ST75 and ST883, have previously been reported to harbour highly divergent alleles of the housekeeping genes used in multilocus sequence typing. Methodology/Principal Findings: ST75-MRSA-IV and ST883-MRSA-IV isolates were characterised in detail. Morphological features as well as 16S sequences were identical to other S. aureus strains. Although a partial rnpB gene sequence was not identical to previously known S. aureus sequences, it was found to be more closely related to S. aureus than to other staphylococci.