chlorogenic acid and rutin play a major role in the in vivo anti-diabetic activity of morus alba leaf extract on type ii diabetic rats绿原酸和芸香苷在体内发挥重要作用的桑属阿尔巴叶提取物治疗ii型糖尿病的老鼠.pdfVIP

Chlorogenic Acid and Rutin Play a Major Role in the In Vivo Anti-Diabetic Activity of Morus alba Leaf Extract on Type II Diabetic Rats 1,2 1,3,4 5 6 ´ ´ 6 Attila Hunyadi *, Ana Martins , Tusty-Jiuan Hsieh , Adrienn Seres , Istvan Zupko 1 Institute of Pharmacognosy, University of Szeged, Szeged, Hungary, 2 COST Action CM0804 (Chemical Biology with Natural Products) of the European Commission, ´ Brussels, Belgium, 3 Grupo de Micobacterias, Unidade de Parasitologia e Microbiologia Medica, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Portugal, 4 Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, Szeged, Hungary, 5 Department of Medical Genetics, Kaohsiung Medical University, Kaohsiung, Taiwan, 6 Department of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, Hungary Abstract The leaves of the white mulberry tree (Morus alba L.) are used worldwide in traditional medicine as anti-diabetics. Various constituents of mulberry leaves, such as iminosugars (i.e. 1-deoxynojirimicin), flavonoids and related compounds, polysaccharides, glycopeptides and ecdysteroids, have been reported to exert anti-diabetic activity, but knowledge about their contribution to the overall activity is limited. The objective of the present work was to determine the in vivo anti- diabetic activity of an extract of mulberry leaves (MA), and to examine to what extent three major constituents, chlorogenic acid, rutin and isoquercitrin, might contribute to the observed activity. Quantities of the three constituents of interest in the extract were determined by using HPLC-DAD. Activity was determined by using a ty