choreography of the transcriptome, photophysiology, and cell cycle of a minimal photoautotroph, prochlorococcus转录组的编排,光生理学,细胞周期的最小光合自养生物,原。球藻.pdfVIP

Choreography of the Transcriptome, Photophysiology, and Cell Cycle of a Minimal Photoautotroph, Prochlorococcus Erik R. Zinser1,2, Debbie Lindell1,3, Zackary I. Johnson1,4, Matthias E. Futschik5,6, Claudia Steglich1,7, 1 8 8 8 1 Maureen L. Coleman , Matthew A. Wright , Trent Rector , Robert Steen , Nathan McNulty , Luke R. Thompson9, Sallie W. Chisholm 1,9* 1 Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America, 2 Department of Microbiology, University of Tennessee, Knoxville, Tennessee, United States of America, 3 Faculty of Biology, Technion-Israel Institute of Technology, Haifa, Israel, 4 Department of Oceanography, University of Hawaii, Honolulu, Hawaii, United States of America, 5 Institute of Theoretical Biology, Humboldt University, Berlin, Germany, 6 Center for Molecular and Structural Biomedicine, University of Algarve, Faro, Portugal, 7 Institute of Biology III, University of Freiburg, Freiburg, Germany, 8 Department of Genetics, Harvard Medical School, Boston, Massachusetts, United States of America, 9 Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America Abstract The marine cyanobacterium Prochlorococcus MED4 has the smallest genome and cell size of all known photosynthetic organisms. Like all phototrophs at temperate latitudes, it experiences predictable daily variation in available light energy which leads to temporal regulation and partitioning of key cellular processes. To better understand the tempo and choreography of this minimal phototroph, we studied the entire transcriptome of the cell over a simulated daily light-dark cycle, and pl