chylomicrons promote intestinal absorption and systemic dissemination of dietary antigen (ovalbumin) in mice乳糜微粒促进肠道吸收和系统性传播饮食抗原(卵白蛋白)的老鼠.pdfVIP

Chylomicrons Promote Intestinal Absorption and Systemic Dissemination of Dietary Antigen (Ovalbumin) in Mice 1,2 1,2 3 1,2 3 Yuehui Wang , Sarbani Ghoshal , Martin Ward , Willem de Villiers , Jerold Woodward , Erik Eckhardt1,2* 1 Department of Internal Medicine, University of Kentucky, Lexington, Kentucky, United States of America, 2 Graduate Center for Nutritional Sciences, University of Kentucky, Lexington, Kentucky, United States of America, 3 Department of Microbiology, Immunology and Molecular Medicine, University of Kentucky, Lexington, Kentucky, United States of America Abstract Background: A small fraction of dietary protein survives enzymatic degradation and is absorbed in potentially antigenic form. This can trigger inflammatory responses in patients with celiac disease or food allergies, but typically induces systemic immunological tolerance (oral tolerance). At present it is not clear how dietary antigens are absorbed. Most food staples, including those with common antigens such as peanuts, eggs, and milk, contain long-chain triglycerides (LCT), which stimulate mesenteric lymph flux and postprandial transport of chylomicrons through mesenteric lymph nodes (MLN) and blood. Most dietary antigens, like ovalbumin (OVA), are emulsifiers, predicting affinity for chylomicrons. We hypothesized that chylomicron formation promotes intestinal absorption and systemic dissemination of dietary antigens. Methodology/Principal Findings: Absorption of OVA into MLN and blood was significantly enhanced when OVA was gavaged into fasted mice together