ciliary neurotrophic factor protects striatal neurons against excitotoxicity by enhancing glial glutamate uptake睫状神经营养因子保护纹状体神经元对会提高胶质谷氨酸摄取.pdfVIP

Ciliary Neurotrophic Factor Protects Striatal Neurons against Excitotoxicity by Enhancing Glial Glutamate Uptake 1 2,3 1 2,3 Corinne Beurrier , Mathilde Faideau , Khaled-Ezaheir Bennouar , Carole Escartin , Lydia Kerkerian-Le 1 2,3 1 Goff , Gilles Bonvento , Paolo Gubellini * ´ ´ ´ ´ 1 Institut de Biologie du Developpement de Marseille-Luminy (IBDML), UMR6216 (Centre National de la Recherche Scientifique/Universite de la Mediterranee), Marseille, ` ´ France, 2 Commissariat a l’Energie Atomique, Institut d’Imagerie Biomedicale (I2BM), Molecular Imaging Research Center (MIRCen), Fontenay-aux-Roses, France, 3 Centre ` National de la Recherche Scientifique, Commissariat a l’Energie Atomique URA2210, Fontenay-aux-Roses, France Abstract Ciliary neurotrophic factor (CNTF) is a potent neuroprotective cytokine in different animal models of glutamate-induced excitotoxicity, although its action mechanisms are still poorly characterized. We tested the hypothesis that an increased function of glial glutamate transporters (GTs) could underlie CNTF-mediated neuroprotection. We show that neuronal loss induced by in vivo striatal injection of the excitotoxin quinolinic acid (QA) was significantly reduced (by ,75%) in CNTF- treated animals. In striatal slices, acute QA application dramatically inhibited corticostriatal field potentials (FPs), whose recovery was signi