changes in gene expression during adaptation of listeria monocytogenes to the soil environment在基因表达变化适应单核细胞增多性李斯特氏菌的土壤环境.pdfVIP

Changes in Gene Expression during Adaptation of Listeria monocytogenes to the Soil Environment 1,2 ´ 1,2 1,2 1,2 1,2 Pascal Piveteau *, Geraldine Depret , Barbara Pivato , Dominique Garmyn , Alain Hartmann ´ 1 Universite de Bourgogne, UMR 1229, Dijon, France, 2 INRA, UMR 1229, Dijon, France Abstract Listeria monocytogenes is a ubiquitous opportunistic pathogen responsible for listeriosis. In order to study the processes underlying its ability to adapt to the soil environment, whole-genome arrays were used to analyse transcriptome modifications 15 minutes, 30 minutes and 18 h after inoculation of L. monocytogenes EGD-e in soil extracts. Growth was observed within the first day of incubation and large numbers were still detected in soil extract and soil microcosms one year after the start of the experiment. Major transcriptional reprofiling was observed. Nutrient acquisition mechanisms (phosphoenolpyruvate-dependent phosphotransferase systems and ABC transporters) and enzymes involved in catabolism of specific carbohydrates (b-glucosidases; chitinases) were prevalent. This is consistent with the overrepresentation of the CodY regulon that suggests that in a nutrient depleted environment, L. monocytogenes recruits its extensive repertoire of transporters to acquire a range of substrates for energy production. Citation: Piveteau P, Depret G, Pivato B, Garmyn D, Hartmann A (2011) Changes in Gene Expression during Adaptation of Listeria monocytogenes to the Soil Environment. PLoS ONE 6(9): e24881. doi:10.1371/journal.pone.0024881 Editor: Holger Bruggemann, Max Planck Institute for Infection Biology, Germany Received May 12, 2011; Ac