candidate olfaction genes identified within the helicoverpa armigera antennal transcriptome候选人嗅觉helicoverpa armigera触角转录组基因识别.pdfVIP

Candidate Olfaction Genes Identified within the Helicoverpa armigera Antennal Transcriptome Yang Liu, Shaohua Gu, Yongjun Zhang, Yuyuan Guo, Guirong Wang* State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China Abstract Antennal olfaction is extremely important for insect survival, mediating key behaviors such as host preference, mate choice, and oviposition site selection. Multiple antennal proteins are involved in olfactory signal transduction pathways. Of these, odorant receptors (ORs) and ionotropic receptors (IRs) confer specificity on olfactory sensory neuron responses. In this study, we identified the olfactory gene repertoire of the economically important agricultural pest moth, Helicoverpa armigera, by assembling the adult male and female antennal transcriptomes. Within the male and female antennal transcriptomes we identified a total of 47 OR candidate genes containing 6 pheromone receptor candidates. Additionally, 12 IR genes as well as 26 odorant-binding proteins and 12 chemosensory proteins were annotated. Our results allow a systematic functional analysis across much of conventional ORs repertoire and newly reported IRs mediating the key olfaction-mediated behaviors of H. armigera. Citation: Liu Y, Gu S, Zhang Y, Guo Y, Wang G (2012) Candidate Olfaction Genes Identified within the Helicoverpa armigera Antennal Transcriptome. PLoS ONE 7(10): e48260. doi:10.1371/journal.pone.0048260 Editor: Hiroaki Matsunami, Duke University, United States of America Received August 7, 2012; Accepted September 21, 2012; Published October 26, 2012 Copyright: 2012 Liu et al. This is an open-access article distributed under the terms of the Creative C