combined saxsem based models of the s. elongatus post-translational circadian oscillator and its interactions with the output his-kinase sasa基于saxsem模式相结合的s elongatus翻译后昼夜振荡器及其交互作用与输出his-kinase莎莎.pdfVIP

Combined SAXS/EM Based Models of the S. elongatus Post-Translational Circadian Oscillator and its Interactions with the Output His-Kinase SasA 1 2 1 3 4 Rekha Pattanayek , Dewight R. Williams , Gian Rossi , Steven Weigand , Tetsuya Mori , Carl H. 2,4 2 1 Johnson , Phoebe L. Stewart , Martin Egli * 1 Department of Biochemistry, School of Medicine, Vanderbilt University, Nashville, Tennessee, United States of America, 2 Department of Molecular Physiology and Biophysics, School of Medicine, Vanderbilt University, Nashville, Tennessee, United States of America, 3 DND-CAT Synchrotron Research Center, Northwestern University, Advanced Photon Source, Argonne National Laboratory, Argonne, Illinois, United States of America, 4 Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, United States of America Abstract The circadian clock in the cyanobacterium Synechococcus elongatus is composed of a post-translational oscillator (PTO) that can be reconstituted in vitro from three different proteins in the presence of ATP and a transcription-translation feedback loop (TTFL). The homo-hexameric KaiC kinase, phosphatase and ATPase alternates between hypo- and hyper- phosphorylated states over the 24-h cycle, with KaiA enhancing phosphorylation, and KaiB antagonizing KaiA and promoting KaiC subunit exchange. SasA is a His kinase that relays output signals from the PTO formed by the three Kai proteins to the TTFL. Although the crystal structures for all three Kai proteins are known, atomic resolution structures of Kai and Kai/SasA protein complexes have remained elusive. Here, we present mode