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choosing and using a plant dna barcode选择和使用植物dna条形码
Review
Choosing and Using a Plant DNA Barcode
1 2 3
Peter M. Hollingsworth *, Sean W. Graham , Damon P. Little
1 Genetics and Conservation Section, Royal Botanic Garden Edinburgh, Edinburgh, United Kingdom, 2 University of British Columbia Botanical Garden and Centre for Plant
Research, Faculty of Land and Food Systems, and Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada, 3 Cullman Program for
Molecular Systematics, The New York Botanical Garden, Bronx, New York, United States of America
I+matK (K. J. Kim et al., unpublished). Various combinations of
Abstract: The main aim of DNA barcoding is to establish these markers were discussed at the 2nd International Barcode of
a shared community resource of DNA sequences that can Life conference in Taipei, but no agreement was reached. The
be used for organismal identification and taxonomic following year, Lahaye et al. [8] proposed that matK alone should
clarification. This approach was successfully pioneered in constitute the plant barcode.
animals using a portion of the cytochrome oxidase 1 (CO1)
One of the biggest challenges in reaching agreement on a plant
mitochondrial gene. In plants, establishing a standardized
barcode was a lack of comparative data encompassing all
DNA barcoding system has been more challenging. In this
candidate markers and a broad taxonom
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