comparative analysis of the global transcriptome of anopheles funestus from mali, west africa比较分析全球转录组的蚊马里、西非.pdfVIP

Comparative Analysis of the Global Transcriptome of Anopheles funestus from Mali, West Africa 1. 1. 1 1 1,2 Andrew C. Serazin , Ali N. Dana , Maureen E. Hillenmeyer , Neil F. Lobo , Mamadou B. Coulibaly , 1 1 1 1 3 Michael B. Willard , Brent W. Harker , Igor V. Sharakhov , Frank H. Collins , Jose M. C. Ribeiro *, Nora J. Besansky1* 1 Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, United States of America, 2 Malaria Research and Training Center, Bamako, Mali, 3 Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, United States of America Abstract Background: Anopheles funestus is a principal vector of malaria across much of tropical Africa and is considered one of the most efficient of its kind, yet studies of this species have lagged behind those of its broadly sympatric congener, An. gambiae. In aid of future genomic sequencing of An. funestus, we explored the whole body transcriptome, derived from mixed stage progeny of wild-caught females from Mali, West Africa. Principal Findings: Here we report the functional annotation and comparative genomics of 2,005 expressed sequence tags (ESTs) from An. funestus, which were assembled with a previous EST set from adult female salivary glands from the same mosquito. The assembled ESTs provided for a nonredundant catalog of 1,035 transcripts excluding mitochondria