comparative analysis of salivary bacterial microbiome diversity in edentulous infants and their mothers or primary care givers using pyrosequencing比较分析唾液细菌微生物多样性的无齿的婴儿和母亲或初级护理人员使用焦磷酸测序.pdfVIP

Comparative Analysis of Salivary Bacterial Microbiome Diversity in Edentulous Infants and Their Mothers or Primary Care Givers Using Pyrosequencing 1 1,2 1 3 4 Kimberly D. Cephas , Juhee Kim , Rose Ann Mathai , Kathleen A. Barry , Scot E. Dowd , Brandon S. Meline5, Kelly S. Swanson1,3* 1 Division of Nutritional Sciences, University of Illinois, Urbana, Illinois, United States of America, 2 Department of Kinesiology and Community Health, University of Illinois, Urbana, Illinois, United States of America, 3 Department of Animal Sciences, University of Illinois, Urbana, Illinois, United States of America, 4 Research and Testing Laboratory and Medical Biofilm Research Institute, Lubbock, Texas, United States of America, 5 Department of Maternal and Child Health, Champaign-Urbana Public Health District, Champaign, Illinois, United States of America Abstract Bacterial contribution to oral disease has been studied in young children, but there is a lack of data addressing the developmental perspective in edentulous infants. Our primary objectives were to use pyrosequencing to phylogenetically characterize the salivary bacterial microbiome of edentulous infants and to make comparisons against their mothers. Saliva samples were collected from 5 edentulous infants (mean age = 4.6 61.2 mo old) and their mothers or primary care givers (mean age = 30.869.5 y old). Salivary DNA was extracted, used to generate DNA amplicons of the V4–V6 hypervariable region of the bacterial 16S rDNA gene, and subjected to 454-pyrosequencing. On average, over 80,000 sequences per sample were generated. High bacterial diversity was noted in the saliva of adults [1012 operational taxonomical units (OTU)