comparative proteomic analysis of differentially expressed proteins in the urine of reservoir hosts of leptospirosis比较蛋白质组学分析差异表达蛋白在尿液的宿主,钩端螺旋体病.pdfVIP

Comparative Proteomic Analysis of Differentially Expressed Proteins in the Urine of Reservoir Hosts of Leptospirosis 1,2 1 1 1 3 Jarlath E. Nally *, Avril M. Monahan , Ian S. Miller , Ruben Bonilla-Santiago , Puneet Souda , Julian P. Whitelegge3 1Veterinary Science Centre, UCD School of Agriculture Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin, Republic of Ireland, 2 UCD Conway Institute of Biomolecular and Biomedical Research, College of Life Sciences, University College Dublin, Belfield, Dublin, Republic of Ireland, 3 The Pasarow Mass Spectrometry Laboratory, Departments of Psychiatry and Biobehavioral Sciences, Chemistry and Biochemistry, and the Neuropsychiatric Institute, University of California, Los Angeles, California, United States of America Abstract Rattus norvegicus is a natural reservoir host for pathogenic species of Leptospira. Experimentally infected rats remain clinically normal, yet persistently excrete large numbers of leptospires from colonized renal tubules via urine, despite a specific host immune response. Whilst persistent renal colonization and shedding is facilitated in part by differential antigen expression by leptospires to evade host immune responses, there is limited understanding of kidney and urinary proteins expressed by the host that facilitates such biological equilibrium. Urine pellets were collected from experimentally infected rats shedding leptospires and compared to urine from non-infected controls spiked with in vitro cultivated leptospires for analysis by 2-D DIGE. Differentially expressed host proteins include membrane metallo endopeptidase, napsin A aspartic peptidase, vacuolar H+ATPase, kidney aminopeptidase and immuno