ck2 phosphorylation of schistosoma mansoni hmgb1 protein regulates its cellular traffic and secretion but not its dna transactionsck2曼氏裂体吸虫hmgb1蛋白的磷酸化调节细胞交通和分泌而不是其dna的事务.pdfVIP

CK2 Phosphorylation of Schistosoma mansoni HMGB1 Protein Regulates Its Cellular Traffic and Secretion but Not Its DNA Transactions . . Isabel Caetano de Abreu da Silva , Vitor Coutinho Carneiro , Renata de Moraes Maciel, Rodrigo Furtado ¤ ´ Madeiro da Costa, Daniel Rodrigues Furtado, Francisco Meirelles Bastos de Oliveira , Mario Alberto ´ Cardoso da Silva-Neto, Franklin David Rumjanek, Marcelo Rosado Fantappie* ´ ´ ˜ Instituto de Bioquımica Medica, Programa de Biologia Molecular e Biotecnologia, Universidade Federal do Rio de Janeiro, Ilha do Fundao, Rio de Janeiro, Brasil Abstract Background: The helminth Schistosoma mansoni parasite resides in mesenteric veins where fecundated female worms lay hundred of eggs daily. Some of the egg antigens are trapped in the liver and induce a vigorous granulomatous response. High Mobility Group Box 1 (HMGB1), a nuclear factor, can also be secreted and act as a cytokine. Schistosome HMGB1 (SmHMGB1) is secreted by the eggs and stimulate the production of key cytokines involved in the pathology of schistosomiasis. Thus, understanding the mechanism of SmHMGB1 release becomes mandatory. Here, we addressed the question of how the nuclear SmHMGB1 can reach the extracellular space. Principal Findings: We showed in vitro and in vivo that CK2 phosphorylation was involved in the nucleocytoplasm