characterisation of early mucosal and neuronal lesions following shigella flexneri infection in human colon描述早期粘膜和神经病变后弗氏志贺菌感染人类结肠.pdfVIP

Characterisation of Early Mucosal and Neuronal Lesions Following Shigella flexneri Infection in Human Colon 1,2,3. 1,2,3. 1,2,3 4 5 Emmanuel Coron , Mathurin Flamant , Philippe Aubert , Thilo Wedel , Thierry Pedron , Eric 2,3 1,2,3 5 1,2,3 Letessier , Jean P. Galmiche , Philippe J. Sansonetti , Michel Neunlist * ´ ´ ´ ˆ 1 INSERM, U913, Nantes, France, 2 Universite de Nantes, Faculte de Medecine, Nantes, France, 3 CHU Nantes, Hotel Dieu, Institut des Maladies de l’Appareil Digestif, ´ ´ ´ ´ Nantes, France, 4 Institute of Anatomy, University of Kiel, Kiel, Germany, 5 Institut Pasteur, Unite de Pathogenie Microbienne Moleculaire Unite INSERM 786, Paris, France Abstract Background: Shigella, an enteroinvasive bacteria induces a major inflammatory response responsible for acute rectocolitis in humans. However, early effect of Shigella flexneri (S. flexneri) infection upon the human mucosa and its microenvironement, in particular the enteric nervous system, remains currently unknown. Therefore, in this study, we sought to characterize ex vivo the early events of shigellosis in a model of human colonic explants. In particular, we aimed at identifying factors produced by S. flexneri and responsible for the lesions of the barri