carbon nanotubes enhance cytotoxicity mediated by human lymphocytes in vitro碳纳米管增强细胞毒性由人类淋巴细胞体外.pdfVIP

Carbon Nanotubes Enhance Cytotoxicity Mediated by Human Lymphocytes In Vitro 1. 2. 2 3 2 4 1 1 Zhao Sun , Zhe Liu , Jie Meng , Jie Meng , Jinhong Duan , Sishen Xie , Xin Lu , Zhaohui Zhu , Chen 3 1 2 2 Wang , Shuchang Chen , Haiyan Xu *, Xian-Da Yang * 1 Peking Union Medical College Hospital, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing, China, 2 Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing, China, 3 National Center of Nanoscience and Technology, Beijing, China, 4 Institute of Physics, Chinese Academy of Sciences, Beijing, China Abstract With the expansion of the potential applications of carbon nanotubes (CNT) in biomedical fields, the toxicity and biocompatibility of CNT have become issues of growing concern. Since the immune system often mediates tissue damage during pathogenesis, it is important to explore whether CNT can trigger cytotoxicity through affecting the immune functions. In the current study, we evaluated the influence of CNT on the cytotoxicity mediated by human lymphocytes in vitro. The results showed that while CNT at low concentrations (0.001 to 0.1 mg/ml) did not cause obvious cell death or apoptosis directly, it enhanced lymphocyte-mediated cytotoxicity against multiple human cell lines. In addition, CNT increased the secretion of IFN-c and TNF-a by the lymphocytes. CNT also upregulated the NF-kB expression in lymphocytes, and the blockage of the NF-kB pathway reduced the lymphocyte-mediated cytotoxicity triggered by CNT. These results suggest that CNT at lo