comprehensive gene and microrna expression profiling reveals a role for micrornas in human liver development全面的基因和微rna表达分析显示小分子核糖核酸的作用在人类肝脏发展.pdfVIP

Comprehensive Gene and microRNA Expression Profiling Reveals a Role for microRNAs in Human Liver Development 1 1 2 2 2 1,4 3 Galit Tzur , Ariel Israel , Asaf Levy , Hila Benjamin , Eti Meiri , Yoel Shufaro , Karen Meir , Elina 1 2 4 2 1 1 Khvalevsky , Yael Spector , Nathan Rojansky , Zvi Bentwich , Benjamin E. Reubinoff , Eithan Galun * 1The Goldyne Savad Institute for Gene Therapy, Hadassah Hebrew University Hospital, Jerusalem, Israel, 2 Rosetta Genomics, Rehovot, Israel, 3 Department of Pathology, Hadassah Hebrew University Hospital, Jerusalem, Israel, 4 Department of Obstetrics and Gynecology, Hadassah Hebrew University Hospital, Jerusalem, Israel Abstract Background and Aims: microRNAs (miRNAs) are small noncoding RNAs that regulate cognate mRNAs post-transcriptionally. miRNAs have been implicated in regulating gene expression in embryonic developmental processes, including proliferation and differentiation. The liver is a multifunctional organ, which undergoes rapid changes during the developmental period and relies on tightly-regulated gene expression. Little is known regarding the complex expression patterns of both mRNAs and miRNAs during the early stages of human liver development, and the role of miRNAs in the regulation of this process has not been studied. The aim of this work was to study the impact of miRNAs on gene expression during early human liver development. Methods: Global gene and miRNA expression were profiled in adult and in 9–12w human embryonic livers, using high- density microarrays and quantitative RT-PCR. Results: Embryonic liver samples ex