deciphering the hybridisation history leading to the lager lineage based on the mosaic genomes of saccharomyces bayanus strains nbrc1948 and cbs380t破解杂交导致啤酒历史沿袭基于镶嵌酿酒bayanus菌株的基因组nbrc1948 cbs380t.pdfVIP

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deciphering the hybridisation history leading to the lager lineage based on the mosaic genomes of saccharomyces bayanus strains nbrc1948 and cbs380t破解杂交导致啤酒历史沿袭基于镶嵌酿酒bayanus菌株的基因组nbrc1948 cbs380t.pdf

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deciphering the hybridisation history leading to the lager lineage based on the mosaic genomes of saccharomyces bayanus strains nbrc1948 and cbs380t破解杂交导致啤酒历史沿袭基于镶嵌酿酒bayanus菌株的基因组nbrc1948 cbs380t

Deciphering the Hybridisation History Leading to the Lager Lineage Based on the Mosaic Genomes of Saccharomyces bayanus Strains NBRC1948 and CBS380T 1,2 3 ´ ´ 1,2 1,2 Huu-Vang Nguyen *, Jean-Luc Legras , Cecile Neuveglise , Claude Gaillardin 1 INRA, UMR1319 Micalis, Jouy-en-Josas, France, 2 AgroParisTech, Micalis, Jouy-en-Josas, France, 3 INRA, UMR1083 Sciences Pour l’CEnologie, Montpellier, France Abstract Saccharomyces bayanus is a yeast species described as one of the two parents of the hybrid brewing yeast S. pastorianus. Strains CBS380T and NBRC1948 have been retained successively as pure-line representatives of S. bayanus. In the present study, sequence analyses confirmed and upgraded our previous finding: S. bayanus type strain CBS380T harbours a mosaic genome. The genome of strain NBRC1948 was also revealed to be mosaic. Both genomes were characterized by amplification and sequencing of different markers, including genes involved in maltotriose utilization or genes detected by array-CGH mapping. Sequence comparisons with public Saccharomyces spp. nucleotide sequences revealed that the CBS380T and NBRC1948 genomes are composed of: a predominant non-cerevisiae genetic background belonging to S. uvarum, a second unidentified species provisionally named S. lagerae, and several introgressed S. cerevisiae fragments. The largest cerevisiae-introgressed DNA common to both genomes totals 70kb in length and is distributed in three contigs, cA, cB and cC. These vary in terms of length and presence of MAL31 or MTY1 (maltotriose-transporter gene). In NBRC1948, two additional cerevisiae-contigs, cD and cE, totaling 12kb in leng