dendritic branching of olfactory bulb mitral and tufted cells regulation by trkb树突分支的嗅球僧帽,trkb簇细胞调节.pdfVIP

Dendritic Branching of Olfactory Bulb Mitral and Tufted Cells: Regulation by TrkB 1 1,2 Fumiaki Imamura , Charles A. Greer * 1 Department of Neurosurgery, Yale University School of Medicine, New Haven, Connecticut, United States of America, 2 Department of Neurobiology, Yale University School of Medicine, New Haven, Connecticut, United States of America Abstract Background: Projection neurons of mammalian olfactory bulb (OB), mitral and tufted cells, have dendrites whose morphologies are specifically differentiated for efficient odor information processing. The apical dendrite extends radially and arborizes in single glomerulus where it receives primary input from olfactory sensory neurons that express the same odor receptor. The lateral dendrites extend horizontally in the external plexiform layer and make reciprocal dendrodendritic synapses with granule cells, which moderate mitral/tufted cell activity. The molecular mechanisms regulating dendritic development of mitral/tufted cells is one of the unsolved important problems in the olfactory system. Here, we focused on TrkB receptors to test the hypothesis that neurotrophin-mediate mechanisms contributed to dendritic differentiation of OB mitral/tufted cells. Principal Findings: With immunohistochemical analysis, we found that the TrkB neurotrophin receptor is expressed by both apical and lateral dendrites of mitral/tufted cells and that expression is evident during the early postnatal days when these dendrites exhibit their most robust growth and differentiation. To examine the effect of TrkB activation on mitral/tufted cell dendritic development, we cultured OB neurons. When BDNF or NT4 were introduced into the cultures, there was a significant increase in the number of prim