dentin sialophosphoprotein (dspp) gene-silencing inhibits key tumorigenic activities in human oral cancer cell line, osc2牙质sialophosphoprotein(dspp)基因沉默抑制肿瘤发生的关键活动在人类口腔癌症细胞系,osc2.pdfVIP

Dentin Sialophosphoprotein (DSPP) Gene-Silencing Inhibits Key Tumorigenic Activities in Human Oral Cancer Cell Line, OSC2 1 1 1 1 3,5 1,4 Rajeshree Joshi , Amany Tawfik , Nneka Edeh , Veronica McCloud , Stephen Looney , Jill Lewis , Stephen Hsu1,4, Kalu U. E. Ogbureke1,2,3,4* 1 Department of Oral Biology, School of Dentistry, Medical College of Georgia, Augusta, Georgia, United States of America, 2 Department of Pathology, School of Medicine, Medical College of Georgia, Augusta, Georgia, United States of America, 3 Department of Oral Health and Diagnostic Sciences, School of Dentistry, Medical College of Georgia, Augusta, Georgia, United States of America, 4 School of Graduate Studies, Medical College of Georgia, Augusta, Georgia, United States of America, 5 Department of Biostatistics, School of Graduate Studies, Medical College of Georgia, Augusta, Georgia, United States of America Abstract Background: We determined recently that dentin sialophosphoprotein (DSPP), a member of the SIBLING (Small integrin- binding ligand N-linked glycoproteins) family of phosphoglycoproteins, is highly upregulated in human oral squamous cell carcinomas (OSCCs) where upregulation is associated with tumor aggressiveness. To investigate the effects of DSPP- silencing on the tumorigenic profiles of the oral cancer cell line, OSC2, short-hairpin RNA (shRNA) interference was employed to silence DSPP in OSC2 cells. Methodology/Principal Findings: Multiple regions of DSPP transcript were targeted for shRNA interference using hDSP- shRNA lentiviral particles designed to silence DSPP gene expression. Control shRNA plasmid encoding a scr