cumulus cells gene expression profiling in terms of oocyte maturity in controlled ovarian hyperstimulation using gnrh agonist or gnrh antagonist积云细胞基因表达分析的卵母细胞成熟卵巢过度刺激控制使用促性腺激素或促性腺激素拮抗剂.pdfVIP

Cumulus Cells Gene Expression Profiling in Terms of Oocyte Maturity in Controlled Ovarian Hyperstimulation Using GnRH Agonist or GnRH Antagonist 1 2,3 2 1 2 ˇ Rok Devjak , Klementina Fon Tacer , Peter Juvan , Irma Virant Klun , Damjana Rozman , Eda Vrtacnik Bokal1* 1 Division of Reproductive Medicine, Department of Obstetrics and Gynecology, University Medical Centre Ljubljana, Ljubljana, Slovenia, 2 Centre for Functional Genomics and Bio-Chips, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia, 3 Institute for Hygiene and Pathology of Animal Nutrition, Veterinary Faculty, University of Ljubljana, Ljubljana, Slovenia Abstract In in vitro fertilization (IVF) cycles controlled ovarian hyperstimulation (COH) is established by gonadotropins in combination with gonadotropin-releasing hormone (GnRH) agonists or antagonists, to prevent premature luteinizing hormone (LH) surge. The aim of our study was to improve the understanding of gene expression profile of cumulus cells (CC) in terms of ovarian stimulation protocol and oocyte maturity. We applied Affymetrix gene expression profiling in CC of oocytes at different maturation stages using either GnRH agonists or GnRH antagonists. Two analyses were performed: the first involved CC of immature metaphase I (MI) and mature metaphase II (MII) oocytes where 359 genes were differentially expressed, and the second involved the two GnRH analogues where no differentially expressed genes were observed at the ¨ entire transcriptome level. A further