coordinated regulation of atf2 by mir-26b in γ-irradiated lung cancer cells协调监管的atf2 mir-26bγ-irradiated肺癌细胞.pdfVIP

Coordinated Regulation of ATF2 by miR-26b in c- Irradiated Lung Cancer Cells . . Himanshu Arora , Rehana Qureshi , Ae-Kyung Park, Woong-Yang Park* Department of Biomedical Sciences, Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, Korea Abstract MicroRNA regulates cellular responses to ionizing radiation (IR) through translational control of target genes. We analyzed time-series changes in microRNA expression following c-irradiation in H1299 lung cancer cells using microarray analysis. Significantly changed IR-responsive microRNAs were selected based on analysis of variance analysis, and predicted target mRNAs were enriched in mitogen-activated protein kinase (MAPK) signaling. Concurrent analysis of time-series mRNA and microRNA profiles uncovered that expression of miR-26b was down regulated, and its target activating transcription factor 2 (ATF2) mRNA was up regulated in c-irradiated H1299 cells. IR in miR-26b overexpressed H1299 cells could not induce expression of ATF2. When c-Jun N-terminal kinase activity was inhibited using SP600125, expression of miR-26b was induced following c-irradiation in H1299 cells. From these results, we concluded that IR-induced up-regulation of ATF2 was coordinately enhanced by suppression of miR-26b in lung cancer cells, which may enhance the effect of IR in the MAPK signaling pathway. Citation: Arora H, Qureshi R, Park A-K, Park W-Y (2011) Coordinated Regulation of ATF2 by miR-26b in c-Irradiated Lung Cancer Cells. PLoS ONE 6(8): e23802. doi:10.1371/journal.pone.0023802 Editor: Sangdun Choi, Ajou University, Korea Received June 16, 2011; Accepted July 26, 2011; Published August 25, 2011 Copyright: 2011 Arora et al. This is an open-access article distributed under the terms