comparative analysis of the recently discovered hat transposon tcbuster in human cells最近发现的比较分析帽子转座子tcbuster在人类细胞.pdfVIP

Comparative Analysis of the Recently Discovered hAT Transposon TcBuster in Human Cells 1 3 4 1 2 2 Lauren E. Woodard , Xianghong Li , Nirav Malani , Aparna Kaja , Robert H. Hice , Peter W. Atkinson , 4 3 1,5 Frederic D. Bushman , Nancy L. Craig , Matthew H. Wilson * 1 Department of Medicine, Division of Nephrology, Baylor College of Medicine, Houston, Texas, United States of America, 2 Department of Entomology Institute for Integrative Genome Biology, University of California Riverside, Riverside, California, United States of America, 3 Howard Hughes Medical Institute, Department of Molecular Biology and Genetics, Johns Hopkins School of Medicine, Baltimore, Maryland, United States of America, 4 Department of Microbiology, Perlman School of Medicine at the University of Pennsylvania, Philadelphia, Pennsylvania, United States of America, 5 Michael E. DeBakey Veteran’s Administration Medical Center, Houston, Texas, United States of America Abstract Background: Transposons are useful tools for creating transgenic organisms, insertional mutagenesis, and genome engineering. TcBuster, a novel hAT-family transposon system derived from the red flour beetle Tribolium castaneum, was shown to be highly active in previous studies in insect embryoes. Methodology/Principal Findings: We tested TcBuster for its activity in human embryonic kidney 293 (HEK-293) cells. Excision footprints obtained from HEK-293 cells contained small insertions and deletions consistent with a hAT-type repair mechanism of hairpin formation and non-homologous end-joining. Genome-wide analysis of