chronic effects of a salmonella type iii secretion effector protein avra in vivo慢性影响沙门氏菌的iii型分泌效应蛋白avra体内.pdfVIP

Chronic Effects of a Salmonella Type III Secretion Effector Protein AvrA In Vivo 1 1 1 2 1 1,3,4 Rong Lu , Shaoping Wu , Xingyin Liu , Yinglin Xia , Yong-guo Zhang , Jun Sun * 1 Department of Medicine, University of Rochester, Rochester, New York, United States of America, 2 Department of Biostatistics and Computational Biology, University of Rochester, Rochester, New York, United States of America, 3 Department of Microbiology and Immunology, University of Rochester, Rochester, New York, United States of America, 4 Wilmot Cancer Center, University of Rochester, Rochester, New York, United States of America Abstract Background: Salmonella infection is a common public health problem that can become chronic and increase the risk of inflammatory bowel diseases and cancer. AvrA is a Salmonella bacterial type III secretion effector protein. Increasing evidence demonstrates that AvrA is a multi-functional enzyme with critical roles in inhibiting inflammation, regulating apoptosis, and enhancing proliferation. However, the chronic effects of Salmonella and effector AvrA in vivo are still unknown. Moreover, alive, mutated, non-invasive Salmonella is used as a vector to specifically target cancer cells. However, studies are lacking on chronic infection with non-pathogenic or mutated Salmonella in the host. Methods/Principal Findings: We infected mice with Salmonella Typhimurium for 27 weeks and investigated the physiological effects as well as the role of AvrA in intestinal inflammation. We found altered body weight, intestinal pathology, and bacterial translocation in spleen, liver, and