comparative analysis of single-species and polybacterial wound biofilms using a quantitative, in vivo, rabbit ear model比较分析单一物种和polybacterial伤口使用定量生物膜,体内,兔耳模型.pdfVIP

Comparative Analysis of Single-Species and Polybacterial Wound Biofilms Using a Quantitative, In Vivo, Rabbit Ear Model 1 1 1 2 1 Akhil K. Seth *, Matthew R. Geringer , Seok J. Hong , Kai P. Leung , Robert D. Galiano , Thomas A. Mustoe1* 1 Division of Plastic Surgery, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America, 2 Microbiology Branch, US Army Dental and Trauma Research Detachment, Institute of Surgical Research, Fort Sam Houston, San Antonio, Texas, United States of America Abstract Introduction: The recent literature suggests that chronic wound biofilms often consist of multiple bacterial species. However, without appropriate in vivo, polybacterial biofilm models, our understanding of these complex infections remains limited. We evaluate and compare the effect of single- and mixed-species biofilm infections on host wound healing dynamics using a quantitative, in vivo, rabbit ear model. Methods: Six-mm dermal punch wounds in New Zealand rabbit ears were inoculated with Staphylococcus aureus strain UAMS-1, Pseudomonas aeruginosa strain PAO1, or both, totaling 10‘6 colony-forming units/wound. Bacterial proliferation and maintenance in vivo were done using procedures from our previously published model. Wounds were harvested for histological measurement of wound healing, viable bacterial counts using selective media, or inflammatory cytokine (IL-1b, TNF-a) expression via quantitative reverse-transcription PCR. Biofilm structure was studied using scanning electron microscopy (SEM). For comparison, biofilm deficient mutant UAMS-929 replaced strain UAMS-1 in some mixed-species infections. Results: Bact