co-ordinated gene expression in the liver and spleen during schistosoma japonicum infection regulates cell migration协调基因表达在肝脏和脾脏在日本血吸虫感染调节细胞迁移.pdfVIP

Co-ordinated Gene Expression in the Liver and Spleen during Schistosoma japonicum Infection Regulates Cell Migration 1,2 1 3 1 1 Melissa L. Burke , Donald P. McManus , Grant A. Ramm , Mary Duke , Yuesheng Li , Malcolm K. 4,5 1 Jones , Geoffrey N. Gobert * 1 Molecular Parasitology Laboratory, Queensland Institute of Medical Research, Herston, Queensland, Australia, 2 The School of Population Health, The University of Queensland, Herston, Queensland, Australia, 3 Hepatic Fibrosis Laboratory, Queensland Institute of Medical Research, Herston, Queensland, Australia, 4 Parasite Cell Biology Laboratory, Queensland Institute of Medical Research, Herston, Queensland, Australia, 5 The School of Veterinary Science, The University of Queensland, St. Lucia, Queensland, Australia Abstract Determining the molecular events induced in the spleen during schistosome infection is an essential step in better understanding the immunopathogenesis of schistosomiasis and the mechanisms by which schistosomes modulate the host immune response. The present study defines the transcriptional and cellular events occurring in the murine spleen during the progression of Schistosoma japonicum infection. Additionally, we compared and contrasted these results with those we have previously reported for the liver. Microarray analysis combined with flow cytometry and histochemistry demonstrated that transcriptional changes occurring in the spleen were closely related to changes in cellular composition. Additionally, the presence of alternatively activated macrophages, as indicated by up-regulation of Chi3l3 and Chi3l4 and expansion of F4/80+ macrophages, together with enhanced expression of the immunor