diagnosis of tuberculosis in the wild boar (sus scrofa) a comparison of methods applicable to hunter-harvested animals诊断肺结核的野猪(野猪)比较的方法适用于hunter-harvested动物.pdfVIP

Diagnosis of Tuberculosis in the Wild Boar (Sus scrofa): A Comparison of Methods Applicable to Hunter-Harvested Animals 1,2,3 4 1 ´ 3 1 Nuno Santos *, Margarida Geraldes , Andreia Afonso , Virgılio Almeida , Margarida Correia-Neves ´ 1 Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal, 2 Pygargus Lda, Povoa Lanhoso, Portugal, 3 Centro de ˜ ´ ´ Investigac¸ao Interdisciplinar em Sanidade Animal – Faculdade de Medicina Veterinaria (CIISA/FMV), TULisbon, Polo Universitario do Alto da Ajuda, Lisbon, Portugal, ˜ 4 National Institute of Biological Resources, Veterinary Research National Laboratory (LNIV), Vairao, Portugal Abstract Background: To obtain robust epidemiological information regarding tuberculosis (TB) in wildlife species, appropriate diagnostic methods need to be used. Wild boar (Sus scrofa) recently emerged as a major maintenance host for TB in some European countries. Nevertheless, no data is available to evaluate TB post-mortem diagnostic methods in hunter-harvested wild boar. Methodology/Principal Findings: Six different diagnostic methods for TB were evaluated in parallel in 167 hunter- harvested wild boar. Compared to bacteriological culture, estimates of sensitivity of histopathology was 77.8%, gross pathology 72.2%, PCR for