denial of reward in the neonate shapes sociability and serotonergic activity in the adult rat拒绝奖励在新生儿形状社交和成年大鼠血清素激活的活动.pdfVIP

Denial of Reward in the Neonate Shapes Sociability and Serotonergic Activity in the Adult Rat 1 1 1 2 Anastasia Diamantopoulou , Androniki Raftogianni , Antonios Stamatakis , Filaretos Alikaridis , 3 1 Melly S. Oitzl , Fotini Stylianopoulou * 1 Laboratory of Biology-Biochemistry, School of Health Sciences, University of Athens, Athens, Greece, 2 Department of Biological Chemistry, School of Medicine, University of Athens, Athens, Greece, 3 Division of Medical Pharmacology, Leiden University, Leiden, The Netherlands Abstract Background: Manipulations of the early environment are linked to long-lasting alterations of emotionality and social capabilities. Denial of rewarding mother-pup interactions in early life of rats could serve as model for child neglect. Negative consequences for social competence in later life, accompanied by changes in the serotonergic system would be expected. In contrast, rewarding mother-pup contact should promote adequate social abilities. Methodology/Principal Findings: Male Wistar rats trained in a T-maze during postnatal days 10–13 under denial (DER) or permission (RER) of maternal contact were tested for play behavior in adolescence and for coping with defeat in adulthood. We estimated serotonin (5-HT) levels in the brain under basal conditions and following defeat, as well as serotonin receptor 1A (5-HT1A) and serotonin transporter (SERT) expression. DER rats exhibited increased aggressive-like play behavior in adolescence (i.e. increased nape attacks, p,0.0001) and selected a proactive coping style during defeat in adulthood (higher sum of proactive behaviors: number of attacks, flights, rear