differential conservation and divergence of fertility genes boule and dazl in the rainbow trout差动保护和散度的生育基因议会和dazl彩虹鳟鱼.pdfVIP

Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout 1. 1. 1 1 1 1 1 Mingyou Li , Qian Shen , Hongyan Xu , Foong Mei Wong , Jianzhou Cui , Zhendong Li , Ni Hong , 1 1 2 1,3 Li Wang , Haobin Zhao , Bo Ma , Yunhan Hong * 1 Department of Biological Sciences, National University of Singapore, Singapore, Singapore, 2 Heilongjiang Fisheries Research Institute, Ha’erbin City, China, 3 Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou, China Abstract Background: The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in invertebrates and mammals but a bisexual mitotic and meiotic expression in medaka. How boule and dazl have evolved different expression patterns in diverse organisms has remained unknown. Methodology and Principal Findings: Here we chose the fish rainbow trout (Oncorhynchus mykiss) as a second lower vertebrate model to investigate the expression of boule and dazl. By molecular cloning and sequence comparison, we identified cDNAs encoding the trout Boule and Dazl proteins, which have a conserved RNA-recognition motif and a maximal similarity to their homologs. By RT-PCR analysis, adult RNA expression of trout boule and dazl is restricted to the gonads of both sexes. By chromogenic and two-color fluorescence in situ hybridization, we revealed bisexual and germline-specific