differential effects of cystathionine-γ-lyase–dependent vasodilatory h2s in periadventitial vasoregulation of rat and mouse aortas微分的影响血管扩张性h2s在cystathionine-γ-lyase-dependent periadventitial vasoregulation大鼠和小鼠主动脉.pdfVIP

Differential Effects of Cystathionine-c-lyase–Dependent Vasodilatory H2S in Periadventitial Vasoregulation of Rat and Mouse Aortas ¨ 1 1 ´ ´ ´ ´ 1 ´ 1 1 Carolin Kohn , Johanna Schleifenbaum , Istvan Andras Szijarto , Lajos Marko , Galyna Dubrovska , 2 1 Yu Huang , Maik Gollasch * ´ ¨ 1 Medical Clinic for Nephrology and Internal Intensive Care, Charite Campus Virchow Klinikum and Experimental and Clinical Research Center (ECRC), Max Delbruck Center for Molecular Medicine, Berlin, Germany, 2 Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences, School of Biomedical Sciences, Chinese University of Hong Kong, Shatin, Hong Kong, China Abstract Background: Hydrogen sulfide (H S) is a potent vasodilator. However, the complex mechanisms of vasoregulation by H S 2 2 are not fully understood. We tested the hypotheses that (1) H2S exerts vasodilatory effects by opening KCNQ-type voltage- dependent (K ) K+ channels and (2) that H S-producing cystathionine-c-lyase (CSE) in perivascular adipose tissue plays a v 2 major role in this pathway. Methodology/Principal Findings: Wire myography of rat and mouse aortas was used. NaHS and 5-(4-hydroxyphenyl)-3H- 1,2-dithiole-3-thione (ADTOH) were used as H2S donors. KCNQ-type Kv channels were blocked by XE991. 4-Propargylglycine (PPG) and ß-cyano-l-alanine (BCA), or 2-(aminooxy)-acetic acid (AOAA) were us