原创力文档detection of mycobacterium ulcerans by the loop mediated isothermal amplification method环介导等温扩增检测溃疡分枝杆菌的方法.pdfVIP
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detection of mycobacterium ulcerans by the loop mediated isothermal amplification method环介导等温扩增检测溃疡分枝杆菌的方法
Detection of Mycobacterium ulcerans by the Loop
Mediated Isothermal Amplification Method
1 1 2 3 4
Anthony Ablordey *, Diana Ackon Amissah , Isaac Frimpong Aboagye , Ben Hatano , Toshio Yamazaki ,
3 5 3
Tetsutaro Sata , Koichi Ishikawa , Harutaka Katano
1 Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana, 2 Department of Animal Science and Conservation
Biology, University of Ghana, Legon, Ghana, 3 Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan, 4 Division of Biosafety Control and
Research, National Institute of Infectious Diseases, Tokyo, Japan, 5 AIDS Research Center, National Institute of Infectious Diseases, Tokyo, Japan
Abstract
Background: Buruli ulcer (BU) caused by Mycobacterium ulcerans (M. ulcerans) has emerged as an important public health
problem in several rural communities in sub-Saharan Africa. Early diagnosis and prompt treatment are important in
preventing disfiguring complications associated with late stages of the disease progression. Presently there is no simple and
rapid test that is appropriate for early diagnosis and use in the low-resource settings where M. ulcerans is most prevalent.
Methodology: We compared conventional and pocket warmer loop mediated isothermal amplification (LAMP) methods
(using a heat block and a pocket warmer respectively as heat source for amplification reaction) for the detection of M.
ulcerans in clinical specimens. The effect of purified and crude DNA preparations on the detection rate of the LAMP assays
were also investigated and
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