dcaf26, an adaptor protein of cul4-based e3, is essential for dna methylation in neurospora crassadcaf26,适配器蛋白质cul4-based e3,粗糙脉孢菌的dna甲基化是至关重要的.pdfVIP

DCAF26, an Adaptor Protein of Cul4-Based E3, Is Essential for DNA Methylation in Neurospora crassa 1 1 1 1 1 1 2 1 Hui Xu , Jiyong Wang , Qiwen Hu , Yun Quan , Huijie Chen , Yingqiong Cao , Chunbo Li , Ying Wang , Qun He1* 1 State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China, 2 Biomedical Analysis Center, Tsinghua University, Beijing, China Abstract DNA methylation is involved in gene silencing and genome stability in organisms from fungi to mammals. Genetic studies in Neurospora crassa previously showed that the CUL4-DDB1 E3 ubiquitin ligase regulates DNA methylation via histone H3K9 trimethylation. However, the substrate-specific adaptors of this ligase that are involved in the process were not known. Here, we show that, among the 16 DDB1- and Cul4-associated factors (DCAFs) encoded in the N. crassa genome, three interacted strongly with CUL4-DDB1 complexes. DNA methylation analyses of dcaf knockout mutants revealed that dcaf26 was required for all of the DNA methylation that we observed. In addition, histone H3K9 trimethylation was also eliminated in dcaf26KO mutants. Based on the finding that DCAF26 associates with DDB1 and the histone methyltransferase DIM-5, we propose that DCAF26 protein is the major adaptor subunit of the Cul4-DDB1-DCAF26 complex, which recruits DIM-5 to DNA regions to initiate H3K9 trimethylation and DNA methylation in N. crassa. Citation: Xu H, Wang J, Hu Q, Quan Y, Chen H, et al. (2010) DCAF26, an Adaptor Protein of Cul4-Based E3, Is Essential for DNA Methylation in Neurospora crassa. PLoS Genet 6(9): e1001132. doi:10.1371/journal.pgen.1001132 Editor: Wendy A. Bickmore,