dazl relieves mirna-mediated repression of germline mrnas by controlling poly(a) tail length in zebrafishdazl缓解mirna-mediated镇压生殖系mrna通过控制聚在斑马鱼(a)尾长.pdfVIP

DAZL Relieves miRNA-Mediated Repression of Germline mRNAs by Controlling Poly(A) Tail Length in Zebrafish 1 1 2 1 1 Yasuaki Takeda , Yuichiro Mishima , Toshinobu Fujiwara , Hiroshi Sakamoto , Kunio Inoue * 1 Department of Biology, Graduate School of Science, Kobe University, Kobe, Japan, 2 Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Kobe, Japan Abstract Background: During zebrafish embryogenesis, microRNA (miRNA) miR-430 contributes to restrict Nanos1 and TDRD7 to primordial germ cells (PGCs) by inducing mRNA deadenylation, mRNA degradation, and translational repression of nanos1 and tdrd7 mRNAs in somatic cells. The nanos1 and tdrd7 39UTRs include cis-acting elements that allow activity in PGCs even in the presence of miRNA-mediated repression. Methodology/Principal Findings: Using a GFP reporter mRNA that was fused with tdrd7 39UTR, we show that a germline- specific RNA-binding protein DAZ-like (DAZL) can relieve the miR-430-mediated repression of tdrd7 mRNA by inducing poly(A) tail elongation (polyadenylation) in zebrafish. We also show that DAZL enhances protein synthesis via the 39UTR of dazl mRNA, another germline mRNA targeted by miR-430. Conclusions/Significance: Our present study indicated that DAZL acts as an ‘‘anti-miRNA factor’’ during vertebrate germ cell development. Our data also suggested that miRNA-mediated regulation can be modulated on specific target mRNAs through the poly(A) tail control. Citation: Takeda Y, Mishima Y, Fujiwara T, Sakamoto H, Inoue K (2009) DAZL Relieves miRNA-Mediated Repression of Germline mRNAs by Controlling Poly(A) Tail Length in Zebrafish. PLoS ONE 4(10): e7513. doi:10.1371/journal.pone.0007513 Editor: Micha