down-regulation of cdh1 is associated with expression of snai1 in colorectal adenomas下调的背景与表达snai1结直肠腺瘤.pdfVIP

Down-Regulation of CDH1 Is Associated with Expression of SNAI1 in Colorectal Adenomas 1. 1. 1 2 1 Feride Kroepil , Georg Fluegen , Zaurbek Totikov , Stephan E. Baldus , Christian Vay , 1 1 1 1 1 Matthias Schauer , Stefan A. Topp , Jan Schulte am Esch , Wolfram T. Knoefel , Nikolas H. Stoecklein * ¨ ¨ ¨ 1 Department of General-, Visceral- and Pediatric Surgery, University Hospital Dusseldorf, Dusseldorf, Germany, 2 Institute for Pathology, University Hospital Dusseldorf, ¨ Dusseldorf, Germany Abstract Introduction: Down-regulation of E-cadherin (CDH1) and epithelial-mesenchymal transition (EMT) are considered critical events for invasion and metastasis of colorectal carcinoma. Here we tested whether the important regulators of E-cadherin expression SNAI1 and TWIST1 are already detectable in human colorectal adenomas. Methods: RNA was extracted from a set of randomly selected formalin-fixed and paraffin-embedded (FFPE) colorectal adenomas (n = 41) and normal colon mucosa (n = 10). Subsequently mRNA expression of CDH1, CDH2, SNAI1 and TWIST1 was analysed by quantitative RT-PCR analysis. CDH1 as well as SNAI1 protein expression were assessed by immunohistochemistry (IHC). Results: SNAI1 mRNA was expressed in 78% (n = 32/41), TWIST1 mRNA in 41% (n = 17/41) and CDH2 mRNA in 41% (n = 17/ 41) of the colorectal adenoma tissue, while normal colon mucosa was