d-ribose induces cellular protein glycation and impairs mouse spatial cognition一小诱发细胞蛋白糖化和空间认知损害老鼠.pdfVIP

D-Ribose Induces Cellular Protein Glycation and Impairs Mouse Spatial Cognition 1,3 1,4 1 1 1,2 Chanshuai Han , Yang Lu , Yan Wei , Ying Liu , Rongqiao He * 1 State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China, 2 Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing, China, 3 Graduate University of the Chinese Academy of Sciences, Beijing, China, 4 School of Life Sciences, University of Science and Technology of China, Anhui, China Abstract Background: D-Ribose, an important reducing monosaccharide, is highly active in the glycation of proteins, and results in the rapid production of advanced glycation end products (AGEs) in vitro. However, whether D-ribose participates in glycation and leads to production of AGEs in vivo still requires investigation. Methodology/Principal Findings: Here we treated cultured cells and mice with D-ribose and D-glucose to compare ribosylation and glucosylation for production of AGEs. Treatment with D-ribose decreased cell viability and induced more AGE accumulation in cells. C57BL/6J mice intraperitoneally injected with D-ribose for 30 days showed high blood levels of glycated proteins and AGEs. Administration of high doses D-ribose also accelerated AGE formation in the mouse brain and induced impairment of spatial learning and memory ability according to the performance in Morris water maze test. Conclusions/Significance: These data demonstrate that D-ribose but not D-glucose reacts rapidly with proteins and produces significant amounts of AGEs in both cultured cells and the mouse brain, leading to accumulation of AGEs which may