drosophila melanogaster as a model host for the burkholderia cepacia complex黑腹果蝇作为这种模式主机不过复杂.pdfVIP

Drosophila melanogaster as a Model Host for the Burkholderia cepacia Complex ´ . . ´ Josee Castonguay-Vanier , Ludovic Vial , Julien Tremblay, Eric Deziel* Institut National de la Recherche Scientifique (INRS)-Institut Armand Frappier, Laval, Canada Abstract Background: Colonization with bacterial species from the Burkholderia cepacia complex (Bcc) is associated with fast health decline among individuals with cystic fibrosis. In order to investigate the virulence of the Bcc, several alternative infection models have been developed. To this end, the fruit fly is increasingly used as surrogate host, and its validity to enhance our understanding of host-pathogen relationships has been demonstrated with a variety of microorganisms. Moreover, its relevance as a suitable alternative to mammalian hosts has been confirmed with vertebrate organisms. Methodology/Principal Findings: The aim of this study was to establish Drosophila melanogaster as a surrogate host for species from the Bcc. While the feeding method proved unsuccessful at killing the flies, the pricking technique did generate mortality within the populations. Results obtained with the fruit fly model are comparable with results obtained using mammalian infection models. Furthermore, validity of the Drosophila infection model was confirmed with B. cenocepacia K56-2 mutants known to be less virulent in murine hosts or in other alternative models. Competitive index (CI) analyses were also performed using the fruit fly as host. Results of CI experiments agree with those obtained with mammalian models. Conclusions/Significance: We conclude that Drosophila is a useful alternative infection model for Bcc and that fly pricking assays and c