drosophila ten-m and filamin affect motor neuron growth cone guidance果蝇ten-m和细丝蛋白影响运动神经元生长锥的指导.pdfVIP

Drosophila Ten-m and Filamin Affect Motor Neuron Growth Cone Guidance 1 2 2 2 1 1,3 Lihua Zheng , Yehudit Michelson , Vita Freger , Ziva Avraham , Koen J. T. Venken , Hugo J. Bellen , 1 2 Monica J. Justice *, Ron Wides * 1 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America, 2 Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel, 3 Program in Developmental Biology, Department of Neuroscience, and Howard Hughes Medical Institute (HHMI), Baylor College of Medicine, Houston, Texas, United States of America Abstract The Drosophila Ten-m (also called Tenascin-major, or odd Oz (odz)) gene has been associated with a pair-rule phenotype. We identified and characterized new alleles of Drosophila Ten-m to establish that this gene is not responsible for segmentation defects but rather causes defects in motor neuron axon routing. In Ten-m mutants the inter-segmental nerve (ISN) often crosses segment boundaries and fasciculates with the ISN in the adjacent segment. Ten-m is expressed in the central nervous system and epidermal stripes during the stages when the growth cones of the neurons that form the ISN navigate to their targets. Over-expression of Ten-m in epidermal cells also leads to ISN misrouting. We also found that Filamin, an actin binding protein, physically interacts with the Ten-m protein. Mutations in cheerio, which encodes Filamin, cause defects in motor neuron axon routing like those of Ten-m. During embryonic development, the expression of Filamin and Ten-m partially overlap in ectodermal cells. These results sugge