early interferon-γ production in human lymphocyte subsets in response to nontyphoidal salmonella demonstrates inherent capacity in innate cells早期interferon-γ生产在人类淋巴细胞子集,以应对nontyphoidal沙门氏菌显示内在固有细胞的能力.pdfVIP

Early Interferon-c Production in Human Lymphocyte Subsets in Response to Nontyphoidal Salmonella Demonstrates Inherent Capacity in Innate Cells 1,2. 1. 2,3 1 Tonney S. Nyirenda , Anna E. Seeley , Wilson L. Mandala , Mark T. Drayson , Calman A. MacLennan1,4* 1 Medical Research Council Centre for Immune Regulation and Clinical Immunology Service, Institute of Biomedical Research, School of Immunity and Infection, College of Medicine and Dental Sciences, University of Birmingham, Birmingham, United Kingdom, 2 Malawi-Liverpool-Wellcome Trust Clinical Research Programme, College of Medicine, University of Malawi, Blantyre, Malawi, 3 Department of Biochemistry, College of Medicine, University of Malawi, Blantyre, Malawi, 4 Division of Medical Microbiology, School of Infection and Host Defence, University of Liverpool, Liverpool, United Kingdom Abstract Background: Nontyphoidal Salmonellae frequently cause life-threatening bacteremia in sub-Saharan Africa. Young children and HIV-infected adults are particularly susceptible. High case-fatality rates and increasing antibiotic resistance require new approaches to the management of this disease. Impaired cellular immunity caused by defects in the T helper 1 pathway lead to intracellular disease with Salmonella that can be countered by IFNc administration. This report identifies the lymphocyte subsets that produce IFNc early in Salmonella infection. Methodology: Intracellular cytokine staining was used to identify IFNc production in blood lymphocyte subsets of ten healthy adults with antibodies to Salmonella (as evidence of immunity to Salmonella), in response to stimulation with live and heat-killed preparations of the D23580 invasive