ecological niche modelling and ndna sequencing support a new, morphologically cryptic beetle species unveiled by dna barcoding生态位建模和ndna测序支持一个新的形态学的甲虫物种dna条码技术推出的.pdfVIP

Ecological Niche Modelling and nDNA Sequencing Support a New, Morphologically Cryptic Beetle Species Unveiled by DNA Barcoding 1 2 1 1,3 Oliver Hawlitschek , Nick Porch , Lars Hendrich , Michael Balke * 1 Department of Entomology, Zoological State Collection, Munich, Germany, 2 School of Life and Environmental Sciences, Deakin University, Melbourne, Australia, 3 GeoBioCenter, Ludwig-Maximilians-University, Munich, Germany Abstract Background: DNA sequencing techniques used to estimate biodiversity, such as DNA barcoding, may reveal cryptic species. However, disagreements between barcoding and morphological data have already led to controversy. Species delimitation should therefore not be based on mtDNA alone. Here, we explore the use of nDNA and bioclimatic modelling in a new species of aquatic beetle revealed by mtDNA sequence data. Methodology/Principal Findings: The aquatic beetle fauna of Australia is characterised by high degrees of endemism, including local radiations such as the genus Antiporus. Antiporus femoralis was previously considered to exist in two disjunct, but morphologically indistinguishable populations in south-western and south-eastern Australia. We constructed a phylogeny of Antiporus and detected a deep split between these populations. Diagnostic characters from the highly variable nuclear protein encoding arginine kinase gene confirmed the presence of two isolated populations. We then used ecological niche modelling to examine the climatic niche characteristics of the two populations. All results support the status of the two populations as distinct species. We describe the south-western species as Antiporus occidentalis sp.