effect of a standardised dietary restriction protocol on multiple laboratory strains of drosophila melanogaster的影响限制饮食标准化协议在多个实验室的黑腹果蝇.pdfVIP

Effect of a Standardised Dietary Restriction Protocol on Multiple Laboratory Strains of Drosophila melanogaster . . Richard C. Grandison , Richard Wong , Timothy M. Bass, Linda Partridge*, Matthew D. W. Piper UCL Institute of Healthy Ageing, Department of Genetics, Evolution and Environment, University College London, London, United Kingdom Abstract Background: Outcomes of lifespan studies in model organisms are particularly susceptible to variations in technical procedures. This is especially true of dietary restriction, which is implemented in many different ways among laboratories. Principal Findings: In this study, we have examined the effect of laboratory stock maintenance, genotype differences and microbial infection on the ability of dietary restriction (DR) to extend life in the fruit fly Drosophila melanogaster. None of these factors block the DR effect. Conclusions: These data lend support to the idea that nutrient restriction genuinely extends lifespan in flies, and that any mechanistic discoveries made with this model are of potential relevance to the determinants of lifespan in other organisms. Citation: Grandison RC, Wong R, Bass TM, Partridge L, Piper MDW (2009) Effect of a Standardised Dietary Restriction Protocol on Multiple Laboratory Strains of Drosophila melanogaster. PLoS ONE 4(1): e4067. doi:10.1371/journal.pone.0004067 Editor: Hiromu Tanimoto, Max-Planck-Institut fuer Neurobiologie, Germany Received October 1, 2008; Accepted December 1, 2008; Published January 1, 2009 Copyright: 2009 Grandison et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.