dramatic increase in glycerol biosynthesis upon oxidative stress in the anaerobic protozoan parasite entamoeba histolytica显著增加甘油生物合成在厌氧氧化应激的原生动物寄生虫痢疾阿米巴.pdfVIP

Dramatic Increase in Glycerol Biosynthesis upon Oxidative Stress in the Anaerobic Protozoan Parasite Entamoeba histolytica 1,2¤ 3 1,4 3 1,5 Afzal Husain , Dan Sato , Ghulam Jeelani , Tomoyoshi Soga , Tomoyoshi Nozaki * 1 Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan, 2 Department of Parasitology, Gunma University Graduate School of Medicine, Maebashi, Japan, 3 Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan, 4 Department of Biochemistry and Integrative Medical Biology, School of Medicine, Keio University, Tokyo, Japan, 5 Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan Abstract Entamoeba histolytica, a microaerophilic enteric protozoan parasite, causes amebic colitis and extra intestinal abscesses in millions of inhabitants of endemic areas. Trophozoites of E. histolytica are exposed to a variety of reactive oxygen and nitrogen species during infection. Since E. histolytica lacks key components of canonical eukaryotic anti-oxidative defense systems, such as catalase and glutathione system, alternative not-yet-identified anti-oxidative defense strategies have been postulated to be operating in E. histolytica. In the present study, we investigated global metabolic responses in E. histolytica in response to H O - and paraquat-mediated oxidative stress by measuring charged metabolites on capillary electrophoresis 2 2 and time-of-flight mass spectrometry. We found that oxidative stress caused drastic modulation of metabolites involved in glycolysis, chitin biosynthesis, and nucleotide and amino acid metabolism. Oxidative stress