elevated antigen-driven il-9 responses are prominent in peanut allergic humans高架抗原驱动il-9反应是著名的花生过敏的人.pdfVIP

Elevated Antigen-Driven IL-9 Responses Are Prominent in Peanut Allergic Humans 1¤ 1 2 1 1,2 Jungang Xie , Larisa C. Lotoski , Rishma Chooniedass , Ruey-Chyi Su , F. Estelle R. Simons , 3 1,2 1 1,2 Joel Liem , Allan B. Becker , Jude Uzonna , Kent T. HayGlass * 1 Department of Immunology, University of Manitoba, Winnipeg, Canada, 2 Department of Pediatrics and Child Health, University of Manitoba, Winnipeg, Canada, 3 Windsor Allergy Asthma Education Centre, Ontario, Canada Abstract Food allergies, and peanut allergy in particular, are leading causes of anaphylactic fatalities worldwide. The immune mechanisms that underlie food allergy remain ill-defined and controversial, in part because studies in humans typically focus on analysis of a limited number of prototypical Th1/Th2 cytokines. Here we determine the kinetics and prevalence of a broad panel of peanut-driven cytokine and chemokine responses in humans with current peanut allergy vs those with stable, naturally occurring clinical tolerance to peanut. Our primary focus is identification of novel indicators of immune dysregulation. Antigen-specific cytokine mRNA and protein responses were elicited in primary culture via peanut or irrelevant antigen (Leishmania extract, milk antigens) mediated stimulation of fresh peripheral blood cells from 40 individuals. Peanut extract exposure in vitro induced a broad panel of responses associated with Th2/Th9-like, Th1-like and Th17-like immu