epigenetic engineering of ribosomal rna genes enhances protein production核糖体rna基因的表观遗传工程提高蛋白质产量.pdfVIP

Epigenetic Engineering of Ribosomal RNA Genes Enhances Protein Production Raffaella Santoro*, Philipp Lienemann, Martin Fussenegger* Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland Abstract Selection of mammalian high-producer cell lines remains a major challenge for the biopharmaceutical manufacturing industry. Ribosomal RNA (rRNA) genes encode the major component of the ribosome but many rRNA gene copies are not transcribed [1–5] due to epigenetic silencing by the nucleolar remodelling complex (NoRC) [6], which may lim