nmda mediated contextual conditioning changes mirna expression门冬氨酸介导上下文条件microrna的表达变化.pdfVIP

NMDA Mediated Contextual Conditioning Changes miRNA Expression 1,2,5 1,3. 4. 4 5 5 Min Jeong Kye , Pierre Neveu , Yong-Seok Lee , Miou Zhou , Judith A. Steen , Mustafa Sahin , 1,2 4 Kenneth S. Kosik * , Alcino J. Silva 1 Neuroscience Research Institute, University of California Santa Barbara, Santa Barbara, California, United States of America, 2 Department of Molecular, Cellular and Developmental Biology, University of California Santa Barbara, Santa Barbara, California, United States of America, 3 Kavli Institute for Theoretical Physics, University of California Santa Barbara, Santa Barbara, California, United States of America, 4 Departments of Neurobiology, Psychiatry and Psychology, Brain Research Institute, Integrative Center for Learning and Memory, David Geffen School of Medicine at University of California Los Angeles, Los Angeles, California, United States of America, 5 The F.M. Kirby Neurobiology Center, Department of Neurology, Children’s Hospital Boston, Harvard Medical School, Boston, Massachusetts, United States of America Abstract We measured the expression of 187 miRNAs using quantitative real time PCR in the hippocampal CA1 region of contextually conditioned mice and cultured embryonic rat hippocampal neurons after neuronal stimulation with either NMDA or bicuculline. Many of the changes in miRNA expression after these three types of stimulation were similar. Surprisingly, the expression level of half of the 187 measured miRNAs was changed in response to contextual conditioning in an NMDA receptor-dependent manner. Genes that control miRNA biogenesis and components of the RISC als