no apparent costs for facultative antibiotic production by the soil bacterium pseudomonas fluorescens pf0-1没有明显的成本兼性抗生素生产的土壤细菌荧光假pf0-1.pdfVIP

No Apparent Costs for Facultative Antibiotic Production by the Soil Bacterium Pseudomonas fluorescens Pf0-1 1 1 1 1 1 Paolina Garbeva *, Olaf Tyc , Mitja N. P. Remus-Emsermann , Annemieke van der Wal , Michiel Vos , Mark Silby2,3, Wietse de Boer1 1 Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, The Netherlands, 2 Department of Biology, University of Massachusetts Dartmouth, North Dartmouth, Massachusetts, United States of America, 3 Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts, United States of America Abstract Background: Many soil-inhabiting bacteria are known to produce secondary metabolites that can suppress microorganisms competing for the same resources. The production of antimicrobial compounds is expected to incur fitness costs for the producing bacteria. Such costs form the basis for models on the co-existence of antibiotic-producing and non-antibiotic producing strains. However, so far studies quantifying the costs of antibiotic production by bacteria are scarce. The current study reports on possible costs, for antibiotic production by Pseudomonas fluorescens Pf0-1, a soil bacterium that is induced to produce a broad-spectrum antibiotic when it is confronted with non-related bacterial competitors or supernatants of their cultures. Methodology and Principal Findings: We measured the possible cost of antibiotic production for Pseudomonas fluorescens Pf0-1 by monitoring changes in growth rate with and without induction of antibiotic production by supernatant of a bacterial competitor, namely Pedobacter sp.. Experiments were performed in liquid as well as on semi-solid media under nutrient-limited condit