nmr structure of hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeasthsp12的nmr结构所需的蛋白质引起的,饮食在酵母restriction-induced延长寿命.pdfVIP

NMR Structure of Hsp12, a Protein Induced by and Required for Dietary Restriction-Induced Lifespan Extension in Yeast 1,2. ` 1. 1 1 1 Andrew P. Herbert , Michele Riesen , Leanne Bloxam , Effie Kosmidou , Brian M. Wareing , 1 2 3 2 1 James R. Johnson , Marie M. Phelan , Stephen R. Pennington , Lu-Yun Lian , Alan Morgan * 1 Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom, 2 NMR Centre for Structural Biology, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom, 3 University College Dublin Conway Institute of Biomedical and Biomolecular Research, University College Dublin, Dublin, Ireland Abstract Dietary restriction (DR) extends lifespan in yeast, worms, flies and mammals, suggesting that it may act via conserved processes. However, the downstream mechanisms by which DR increases lifespan remain unclear. We used a gel based proteomic strategy to identify proteins whose expression was induced by DR in yeast and thus may correlate with longevity. One protein up-regulated by DR was Hsp12, a small heat shock protein induced by various manipulations known to retard ageing. Lifespan extension by growth on 0.5% glucose (DR) was abolished in an hsp12D strain, indicating that Hsp12 is essential for the longevity effect of DR. In contrast, deletion of HSP12 had no effect on growth under DR conditions or a variety of environmental stresses, indicating that the effect of Hsp12 on lifespan is not due to increased general stress resistance. Unl