non-small cell lung cancer cells expressing cd44 are enriched for stem cell-like properties非小细胞肺癌细胞cd44表达丰富的干细胞的特性.pdfVIP

Non-Small Cell Lung Cancer Cells Expressing CD44 Are Enriched for Stem Cell-Like Properties 1 2,3 4 1 5 Elaine Lai-Han Leung , Ronald R. Fiscus , James W. Tung , Vicky Pui-Chi Tin , Lik Cheung Cheng , Alan 5 4 4,6 1 Dart-Loon Sihoe , Louis M. Fink , Yupo Ma , Maria Pik Wong * 1 Department of Pathology, The University of Hong Kong, Hong Kong, Special Administrative Region, China, 2 Cancer Molecular Biology Section, Nevada Cancer Institute, Las Vegas, Nevada, United States of America, 3 College of Pharmacy, University of Southern Nevada, Henderson, Nevada, United States of America, 4 Division of Laboratory Medicine, Nevada Cancer Institute, Las Vegas, Nevada, United States of America, 5 Cardiothoracic Surgery Unit, Queen Mary Hospital, Hong Kong, Special Administrative Region, China, 6 Department of Pathology, Stony Brook University Medical Center, Stony Brook, New York, United States of America Abstract Background: The cancer stem cell theory hypothesizes that cancers are perpetuated by cancer stem cells (CSC) or tumor initiating cells (TIC) possessing self-renewal and other stem cell-like properties while differentiated non-stem/initiating cells have a finite life span. To investigate whether the hypothesis is applicable to lung cancer, identification of lung CSC and demonstration of these capacities is essential. Methodology/Principal Finding: The expression profiles of five stem cell markers (CD34, CD44, CD133, BMI1 and OCT4) were screened by flow cytometry in 10 lung cancer cell lines. CD44 was further investigated by testing for in vitro and in vivo tumorigenecity. Formation of spheroid bodies