non-sterilized fermentative production of polymer-grade l-lactic acid by a newly isolated thermophilic strain bacillus sp. 2–6non-sterilized发酵生产聚合级l-lactic酸的新孤立的嗜热芽孢杆菌sp。2 - 6.pdfVIP

Non-Sterilized Fermentative Production of Polymer- Grade L-Lactic Acid by a Newly Isolated Thermophilic Strain Bacillus sp. 2–6 1,3 2 1,3 1,2 2 1,2 3 Jiayang Qin , Bo Zhao , Xiuwen Wang , Limin Wang , Bo Yu , Yanhe Ma , Cuiqing Ma , Hongzhi 4 1 1,3,4 Tang , Jibin Sun , Ping Xu * 1Tianjin Industrial Biotechnology RD Center, Chinese Academy of Sciences, Tianjin, People’s Republic of China, 2 Institute of Microbiology, Chinese Academy of Sciences, Beijing, People’s Republic of China, 3 State Key Laboratory of Microbial Technology, Shandong University, Jinan, People’s Republic of China, 4 Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, People’s Republic of China Abstract Background: The demand for lactic acid has been increasing considerably because of its use as a monomer for the synthesis of polylactic acid (PLA), which is a promising and environment-friendly alternative to plastics derived from petrochemicals. Optically pure L-lactic acid is essential for polymerization of PLA. The high fermentation cost of L-lactic acid is another limitation for PLA polymers to compete with conventional plastics. Methodology/Principal Findings: A Bacillus sp. strain 2–6 for production of L-lactic acid was isolated at 55uC from soil samples. Its thermophilic characteristic made it a good lactic acid producer because optically pure L-lactic acid could be produced by this strain under open condition without sterilization. In 5-liter batch fermentation of Bacillus sp. 2–6, 118.0 g/ liter of L-lactic acid with an optical purity of 99.4% was obtained fr