notch and prospero repress proliferation following cyclin e overexpression in the drosophila bristle lineage切口和普洛斯彼罗抑制增殖细胞周期素e过度果蝇刚毛血统.pdfVIP

Notch and Prospero Repress Proliferation following Cyclin E Overexpression in the Drosophila Bristle Lineage 1,2 1,2¤ 1,2 ` 1,2 Franc¸oise Simon , Pierre Fichelson , Michel Gho , Agnes Audibert * ´ 1 Universite Pierre et Marie Curie-Paris 6, UMR 7622, Paris, France, 2 CNRS, UMR 7622, Paris, France Abstract Understanding the mechanisms that coordinate cell proliferation, cell cycle arrest, and cell differentiation is essential to address the problem of how ‘‘normal’’ versus pathological developmental processes take place. In the bristle lineage of the adult fly, we have tested the capacity of post-mitotic cells to re-enter the cell cycle in response to the overexpression of cyclin E. We show that only terminal cells in which the identity is independent of Notch pathway undergo extra divisions after CycE overexpression. Our analysis shows that the responsiveness of cells to forced proliferation depends on both Prospero, a fate determinant, and on the level of Notch pathway activity. Our results demonstrate that the terminal quiescent state and differentiation are regulated by two parallel mechanisms acting simultaneously on fate acquisition and cell cycle progression. Citation: Simon F, Fichelson P, Gho M, Audibert A (2009) Notch and Prospero Repress Proliferation following Cyclin E Overexpression in the Drosophila Bristle Lineage. PLoS Genet 5(8): e1000594. doi:10.1371/journal.pgen.1000594 Editor: Orna Cohen-Fix, National Institute of Diabetes and Digestive and Kidney Diseases, United States of America Received February 18, 2009; Accepted July 10, 2009; Published August 7, 2009 Copyright: 2009 Simon et al. This is an open-access article distributed unde