nuclear progesterone receptors are up-regulated by estrogens in neurons and radial glial progenitors in the brain of zebrafish核雌激素孕激素受体上调的神经元和径向神经胶质祖细胞在斑马鱼的大脑.pdfVIP

Nuclear Progesterone Receptors Are Up-Regulated by Estrogens in Neurons and Radial Glial Progenitors in the Brain of Zebrafish 1 1 1 1 1 Nicolas Diotel , Arianna Servili , Marie-Madeleine Gueguen , Svetlana Mironov , Elisabeth Pellegrini , 1 2 1 1 Colette Vaillant , Yong Zhu , Olivier Kah *, Isabelle Anglade ´ 1 Neurogenesis and Oestrogens, UMR CNRS 6026, IFR140, Universite de Rennes 1, Rennes, France, 2 Department of Biology, East Carolina University, Greenville, North Carolina, United States of America Abstract In rodents, there is increasing evidence that nuclear progesterone receptors are transiently expressed in many regions of the developing brain, notably outside the hypothalamus. This suggests that progesterone and/or its metabolites could be involved in functions not related to reproduction, particularly in neurodevelopment. In this context, the adult fish brain is of particular interest, as it exhibits constant growth and high neurogenic activity that is supported by radial glia progenitors. However, although synthesis of neuroprogestagens has been documented recently in the brain of zebrafish, information on the presence of progesterone receptors is very limited. In zebrafish, a single nuclear progesterone receptor (pgr) has been cloned and characterized. Here, we demonstrate that this pgr is widely distributed in all regions of the zebrafish brain. Interestingly, we show that Pgr is strongly expressed in radial glial cells and more weakly in neurons. Finally, we present evidence, based on quantitative PCR and immunohistochemistry, that nucl